Sometimes we generate large datasets in our efforts to understand metabolism. We have tried to make the data available to our colleagues below.
COLD-ACTIVATED LIPID DYNAMICS IN ADIPOSE TISSUE HIGHLIGHTS A ROLE FOR CARDIOLIPIN IN THERMOGENIC METABOLISM
We measured cold-activated lipid landscapes in human circulation and in mouse adipose tissue by shotgun lipidomics. We created an interactive online viewer to visualize the changes of specific lipid species in response to cold that you can use to see how the concentration of approximately 1,600 different lipid species change in the context of cold exposure. This work was performed in the laboratory of Dr. Yu-Hua Tseng at the Joslin Diabetes Center in Boston, MA, USA.
ADIPOCYTES IN CULTURE
Together with Yu-Hua Tseng and Lee Rubin's Lab at Harvard, we studied mouse and human adipocytes in culture using time-lapse microscopy. Some of these results were published in the Xue et al., Nat Med 2015. A collection of the unpublished data from these studies can be found below.
FFA-LUC IN TISSUE SPECIFIC LUCIFERASE REPORTER MICE
We have used Cre-Lox technology combined with non-invasive bioluminescent imaging to visualize lipid uptake in live mice. We have demonstrated this technique in adipose, muscle and liver tissue. These studies utilize the FFA-SS-Luc substrate from SwissLumix.
TRPV1 LINEAGE MAPS
We have used Cre-Lox technology combined with fluorescent imaging to visualize the cellular lineage of Trpv1 expressing cells in mice. We mapped the Trpv1 lineage in adipose tissue.